Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:1000Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BAG3 antibody [N1C1] diluted at 1:500.| Product Name | BAG3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BAG3 (BCL2-associated athanogene 3)BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BAG3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Rhesus Monkey, BovineMouse (85%), Rhesus Monkey (97%), Bovine (83%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAG-3 antibody BIS antibody CAIR-1 antibody MGC104307 antibody BAG3 antibody docking protein CAIR-1 antibody bcl-2-binding protein Bis antibody BAG family molecular chaperone regulator 3 antibody bcl-2-associated athanogene 3 antibody BAG-family m |
|---|---|
| Molecular Weight(MW) | 62 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:1000
Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BAG3 antibody [N1C1] diluted at 1:500.| Positive Control | U87-MG , MCF-7 , rat heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
