Application
Sample (30 ug of whole cell lysate) A: Hela B: Molt-4 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BAG5 antibody at 1:200 dilution.| Product Name | BAG5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BAG5 (BCL2-associated athanogene 5)The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BAG5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Chimpanzee, BovineMouse (92%), Rat (91%), Chicken (81%), Chimpanzee (99%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAG-5 antibody BAG5 antibody BAG-family molecular chaperone regulator-5 antibody BAG family molecular chaperone regulator 5 antibody bcl-2-associated athanogene 5 antibody BCL2-associated athanogene 5 antibody |
|---|---|
| Target | BAG5 |
| Molecular Weight(MW) | 51 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A: Hela B: Molt-4 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BAG5 antibody at 1:200 dilution.| Positive Control | 293T , HeLa , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
