Application
Sample (50 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: SK-N-SH 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using BAIAP2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
BAIAP2 antibody detects BAIAP2 protein at cytoplasm in Purkinje cells of mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BAIAP2 antibody diluted at 1:500.| Product Name | BAIAP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BAIAP2 (BAI1-associated protein 2)The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BAIAP2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (98%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAP2 antibody FLAF3 antibody IRSP53 antibody BAIAP2 antibody insulin receptor substrate p53/p58 antibody insulin receptor substrate protein of 53 kDa antibody IRSp53/58 antibody IRS-58 antibody brain-specific angiogenesis inhibitor 1-associated pro |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (5) |
| Cellular Localization | Cytoplasm , Membrane , Cell projection , filopodium , ruffle |
Application
Sample (50 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: SK-N-SH 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using BAIAP2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
BAIAP2 antibody detects BAIAP2 protein at cytoplasm in Purkinje cells of mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BAIAP2 antibody diluted at 1:500.| Positive Control | SK-N-SH , mouse brain , *C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
