Application
Immunohistochemical analysis of paraffin-embedded human liver, bile duct, using BAK1 antibody(10 μg/ml).Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 C: Molt-4 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | BAK antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BAK (BCL2-antagonist/killer 1)The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human BAK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BAK antibody BAK-LIKE antibody BCL2L7 antibody CDN1 antibody MGC117255 antibody MGC3887 antibody BAK1 antibody bcl-2-like protein 7 antibody bcl2-L-7 antibody apoptosis regulator BAK antibody BCL2-like 7 protein antibody pro-apoptotic protein BA |
|---|---|
| Molecular Weight(MW) | 23 kDa (note) |
| Cellular Localization | Membrane |
Application
Immunohistochemical analysis of paraffin-embedded human liver, bile duct, using BAK1 antibody(10 μg/ml).
Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 C: Molt-4 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
