Application
Detection of human Bak by immunoblotting. Sample: Whole cell lysates from untreated HL-60 cells. Primary antibody: Anti-Bak (Ab-1) Mouse mAb (TC-100) (2 µg/ml). Detection: Chemiluminescence.| Product Name | Bak antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [TC100] to BakApoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl2 family members, among others, play an important role.The Bcl2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. |
| Immunogen | Recombinant fragment (C-terminal transmembrane portion deleted) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | TC100 |
| Host Species | Mouse |
| Tested Applications | IHC-FWB |
| IHC-Fr: Use at a concentration of 1 - 5 μg/ml. WB: Use at a concentration of 2 - 5 μg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 23.4 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 578 BCL2L7 600516 CDN1 BAK Q16611 BAK1 MGC117255 |
|---|---|
| Target | Bak |
Application
Detection of human Bak by immunoblotting. Sample: Whole cell lysates from untreated HL-60 cells. Primary antibody: Anti-Bak (Ab-1) Mouse mAb (TC-100) (2 µg/ml). Detection: Chemiluminescence.| Positive Control | Tonsil. |
|---|---|
| Application Notes | IHC-Fr: Use at a concentration of 1 - 5 μg/ml. WB: Use at a concentration of 2 - 5 μg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 23.4 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: 0.2% Gelatin, 50mM Sodium Phosphate |
