| Product Name | Band3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [BIII 136] to Band3The Band 3 protein is the most abundant integral protein of human erythrocyte membranes and functions as the major anion transporter polypeptide of erythrocytes. Band 3 is a 90-100 kD protein, its microheterogeneity is ascribed mainly to the structural heterogeneity of the carbohydrate moiety. The C-terminal portion of the molecule spans the membrane several times, and is responsible for anion transport. The N-terminal portion, comprising almost half of the polypeptide chain of Band 3, is located inside the red cell and interacts with cytoskeletal and cytoplasmic proteins such as ankyrin. |
| Immunogen | Full length native protein (purified). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | BIII 136 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIPWB |
| For ELISA: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. Immunofluorescent staining of fixed human erythrocytes. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. The antibody titer (1:5,000) was determined by indirect immunoblotting using an extract of human erythrocyte ghosts. This antibody recognizes Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD. Digestion and aggregation of the Band 3 protein results in its detection with multiple molecular weight proteins. Since the epitope is not located at the erythrocyte surface, the antibody does not agglutinate red blood cells and its binding to the cell surface cannot be detected by an indirect agglutination assay. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Band3 MGC126619 MGC116750 MGC126623 109270 Band 3 P02730 BND3 MGC116753 CD233 DI SLC4A1 WD 6521 EMPB3 WD1 AE1 EPB3 SW RTA1A FR WR |
|---|---|
| Target | Band3 |
| Tissue Specificity | This antibody was found to be specific for the cytoplasmic amino-terminal protein of band 3. This antibody recognizes Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD.Digestion and aggregation of the Band 3 protein results in its detection with multiple molecular weight proteins.This antibody does not react with human fibroblast extract (nonerythroid). |
| Cellular Localization | Cell Membrane and Cytoplasmic |
| Application Notes | For ELISA: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. Immunofluorescent staining of fixed human erythrocytes. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. The antibody titer (1:5,000) was determined by indirect immunoblotting using an extract of human erythrocyte ghosts. This antibody recognizes Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD. Digestion and aggregation of the Band 3 protein results in its detection with multiple molecular weight proteins. Since the epitope is not located at the erythrocyte surface, the antibody does not agglutinate red blood cells and its binding to the cell surface cannot be detected by an indirect agglutination assay. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Contains 0.1% sodium azide |
