Application
BARD1 antibody detects BARD1 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. BARD1 antibody diluted at 1:400.Application
BARD1 antibody detects BARD1 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BARD1 protein stained by BARD1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
BARD1 antibody detects BARD1 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BARD1 antibody diluted at 1:400.Application
A431 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BARD1 antibody diluted at 1:1000.| Product Name | BARD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BARD1 (BRCA1 associated RING domain 1)This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BARD1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BARD-1 antibody BARD1 antibody BARD1 antibody BRCA1 associated RING domain 1 antibody |
|---|---|
| Target | BARD1 |
| Molecular Weight(MW) | 87 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
BARD1 antibody detects BARD1 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. BARD1 antibody diluted at 1:400.
Application
BARD1 antibody detects BARD1 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BARD1 protein stained by BARD1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
BARD1 antibody detects BARD1 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BARD1 antibody diluted at 1:400.
Application
A431 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BARD1 antibody diluted at 1:1000.| Positive Control | A431 , A431 nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
