Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE diluted at 1:1000Application
BAT3 antibody [N1N2], N-term detects BAT3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BAT3 protein stained by BAT3 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | BAT3 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BAT3 (HLA-B associated transcript 3)This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human BAT3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (97%), Rat (96%), Pig (98%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAG-6 antibody BAT3 antibody D6S52E antibody G3 antibody BAG6 antibody protein Scythe antibody protein G3 antibody BAG family molecular chaperone regulator 6 antibody large proline-rich protein BAT3 antibody HLA-B-associated transcript 3 antibody |
|---|---|
| Molecular Weight(MW) | 119 kDa (note) (7) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE diluted at 1:1000
Application
BAT3 antibody [N1N2], N-term detects BAT3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BAT3 protein stained by BAT3 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
