Application
Bax antibody [N1N2], N-term detects Bax protein at cytoplasm, nucleus and mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bax protein stained by Bax antibody [N1N2], N-term diluted at 1:400. Blue: Hoechst 33342 staining.Application
Sample(30 µg whole cell lysate) A:Hep G2 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Bax antibody [N1N2], N-term detects Bax protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Bax antibody [N1N2], N-term diluted at 1:500.Application
Bax antibody [N1N2], N-term immunoprecipitates BCL2-associated X protein in IP experiments. IP samples: MCF-7 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of BCL2-associated X protein by 4 μg Bax antibody [N1N2], N-term 15 % SDS-PAGE The immunoprecipitated BCL2-associated X protein was detected by Bax antibody [N1N2], N-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | Bax antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BAX (BCL2-associated X protein)The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bax. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Dog, Rhesus Monkey, BovineDog (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BCL2L4 antibody BAX antibody bcl-2-like protein 4 antibody apoptosis regulator BAX antibody bcl2-L-4 antibody BCL2-associated X protein antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) (5) |
| Cellular Localization | Isoform Alpha: Mitochondrion membrane , Cytoplasm , Isoform Beta: Cytoplasm , Isoform Gamma: Cytoplasm , Isoform Delta: Cytoplasm |
Application
Bax antibody [N1N2], N-term detects Bax protein at cytoplasm, nucleus and mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bax protein stained by Bax antibody [N1N2], N-term diluted at 1:400. Blue: Hoechst 33342 staining.
Application
Sample(30 µg whole cell lysate) A:Hep G2 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Bax antibody [N1N2], N-term detects Bax protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Bax antibody [N1N2], N-term diluted at 1:500.
Application
Bax antibody [N1N2], N-term immunoprecipitates BCL2-associated X protein in IP experiments. IP samples: MCF-7 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of BCL2-associated X protein by 4 μg Bax antibody [N1N2], N-term 15 % SDS-PAGE The immunoprecipitated BCL2-associated X protein was detected by Bax antibody [N1N2], N-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Positive Control | 293T , HeLaS3 , HepG2 , Molt-4 , Raji , *U2OS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
