Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:500Application
BBOX1 antibody detects BBOX1 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BBOX1 antibody diluted at 1:500.Application
Sample(30 μg of whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:500Application
BBOX1 antibody detects BBOX1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. BBOX1 antibody dilution: 1:500.Application
Immunofluorescence analysis of methanol-fixed A431, using BBOX1 antibody at 1:500 dilution.| Product Name | BBOX1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BBOX1 (butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1)This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BBOX1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (85%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BBH antibody BBOX antibody G-BBH antibody gamma-BBH antibody BBOX1 antibody gamma-butyrobetaine hydroxylase antibody gamma-butyrobetaine 2-oxoglutarate dioxygenase 1 antibody gamma-butyrobetaine dioxygenase antibody butyrobetaine (gamma) 2-oxoglut |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:500
Application
BBOX1 antibody detects BBOX1 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BBOX1 antibody diluted at 1:500.
Application
Sample(30 μg of whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:500
Application
BBOX1 antibody detects BBOX1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. BBOX1 antibody dilution: 1:500.
Application
Immunofluorescence analysis of methanol-fixed A431, using BBOX1 antibody at 1:500 dilution.| Positive Control | Molt-4 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
