Application
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 μg/mL.Application
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | BCAR3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCAR3Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| Immunogen | BCAR3 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BCAR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB: 1 - 2 ug/ml. IF: 20 ug/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | breast cancer anti-estrogen resistance 3 SH2D3B NSP2 |
|---|---|
| Target | BCAR3 |
| Tissue Specificity | At least four isoforms of BCAR3 are known to exist; this antibody will only recognize the largest isoform. BCAR3 antibody is predicted to not cross-react with other BCAR proteins. |
Application
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 μg/mL.
Application
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 μg/mL.| Application Notes | WB: 1 - 2 ug/ml. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stored At 4ºc For Three Months And -20ºc, Stable For Up To One Year. As With All Antibodies Care Should Be Taken To Avoid Repeated Freeze Thaw Cycles. Antibodies Should Not Be Exposed To Prolonged High Temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
