Application
BCCIP antibody detects BCCIP protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. BCCIP antibody dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: A431 B: Hela 10% SDS PAGE diluted at 1:1000Application
BCCIP antibody detects BCCIP protein at nucleus on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. BCCIP antibody dilution: 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BCCIP antibody at 1:200 dilution.| Product Name | BCCIP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCCIP (BRCA2 and CDKN1A interacting protein)This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BCCIP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TOK-1 antibody TOK1 antibody BCCIP antibody TOK-1alpha antibody BRCA2 and Cip1/p21 interacting protein antibody BRCA2 and CDKN1A-interacting protein antibody BCCIPbeta antibody TOK-1beta antibody cdk inhibitor p21 binding protein antibody protein |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
BCCIP antibody detects BCCIP protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. BCCIP antibody dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: A431 B: Hela 10% SDS PAGE diluted at 1:1000
Application
BCCIP antibody detects BCCIP protein at nucleus on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. BCCIP antibody dilution: 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BCCIP antibody at 1:200 dilution.| Positive Control | A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
