Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using BCL-2 (phospho-Thr56) antibody .Application
Western blot analysis of extracts from MDA435 cells treated with sorbtiol (0.4M, 30min) using Bcl-2 (phospho-Thr56) antibody .| Product Name | Bcl-2 (phospho Thr56) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Bcl-2 (phospho Thr56)Bcl2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl2, such as in the case of translocation of Bcl2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C terminal ends. |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 56 (G-H-TP-P-H). |
| Modification | p-Thr56 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| WB: Use at a dilution of 1:500-1:1000 IHC: Use at a dilution of 1:50-1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Apoptosis regulator Bcl 2 Bcl-2 phospho Thr56 Bcl 2 B cell CLL/lymphoma 2 Bcl2 phosphoT56 Bcl2 phospho Thr56 BCL2 protein B cell leukemia/lymphoma 2 Bcl2 phospho T56 Bcl-2 phosphoT56 Apoptosis regulator Bcl2 Bcl-2 phospho T56 B cell leukemia/ly |
|---|---|
| Target | Bcl-2 (phospho Thr56) |
| Molecular Weight(MW) | 26 (note) |
| Tissue Specificity | Human Bcl-2 phospho Thr56 |
| Cellular Localization | Endoplasmic |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using BCL-2 (phospho-Thr56) antibody .
Application
Western blot analysis of extracts from MDA435 cells treated with sorbtiol (0.4M, 30min) using Bcl-2 (phospho-Thr56) antibody .| Application Notes | WB: Use at a dilution of 1:500-1:1000 IHC: Use at a dilution of 1:50-1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol |
