Rabbit Polyclonal antibody to Bcl-2This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]
Immunogen
Bcl-2 antibody was raised against a peptide corresponding to 14 amino acids near the middle of human Bcl-2.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAICC/IFWB
WB: 1 - 4 ug/ml. ICC: 2 ug/ml. IF: 2 ug/ml
Species Reactivity
Mouse
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
B-cell CLL/lymphoma 2 , BCL2 , PPP1R50
Target
Bcl-2
Application
Application
Immunocytochemistry of Bcl-2 in A20 cells with Bcl-2 antibody at 2 ug/mL.
Application
Western blot analysis of Bcl-2 in A-20 cell lysates with Bcl-2 antibody at (A) 1, (B) 2, and (C) 4 ug/mL.
Application Notes
WB: 1 - 4 ug/ml. ICC: 2 ug/ml. IF: 2 ug/ml
Additional Information
Form
Liquid
Storage Instructions
Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.