Application
Formalin fixed paraffin embedded human tonsil stained withApplication
Western blot analysis of human lung cell extract with Bcl2, Antibody.| Product Name | Bcl-2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [124] to Bcl-2Bcl-2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). [Provided by RefSeq] |
| Immunogen | Recombinant Bcl-2 peptide sequence comprising 41 - 54 amino acids of Bcl-2 oncoprotein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 124 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-PIPWB |
| Immunohistochemistry: Use at a dilution of 1:15 - 1:50 for 60 mins at RT (ABC method). : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BCL2 P10415 151430 596 Bcl-2 Bcl 2 |
|---|---|
| Target | Bcl-2 |
| Tissue Specificity | is specific for Bcl-2. In lymphoid tissues, this antibody stains B lymphocytes as well as T cells. |
| Cellular Localization | Endoplasmic reticulum, outer mitochondrial membrane and nuclear membrane. |
| Light Chain Type | kappa |
Application
Formalin fixed paraffin embedded human tonsil stained with
Application
Western blot analysis of human lung cell extract with Bcl2, Antibody.| Positive Control | Tonsil, Human lung cell line |
|---|---|
| Application Notes | Immunohistochemistry: Use at a dilution of 1:15 - 1:50 for 60 mins at RT (ABC method). : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: Sodium Azide; Constituents: Tissue Culture Supernatant, 6M Urea |
