Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using BCL2L10 antibody at 1:200 dilution.Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL2L10 antibody [N2C2], Internal diluted at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using BCL2L10 antibody at 1:100 dilution.| Product Name | BCL2L10 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BCL2L10 (BCL2-like 10 (apoptosis facilitator))The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains conserved BH4, BH1 and BH2 domains. This protein can interact with other members of BCL-2 protein family including BCL2, BCL2L1/BCL-X(L), and BAX. Overexpression of this gene has been shown to suppress cell apoptosis possibly through the prevention of cytochrome C release from the mitochondria, and thus activating caspase-3 activation. The mouse counterpart of this protein is found to interact with Apaf1 and forms a protein complex with Caspase 9, which suggests the involvement of this protein in APAF1 and CASPASE 9 related apoptotic pathway. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BCL2L10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCL-B antibody Boo antibody Diva antibody MGC129810 antibody MGC129811 antibody BCL2L10 antibody anti-apoptotic protein NrH antibody apoptosis regulator Bcl-B antibody bcl2-L-10 antibody bcl-2-like protein 10 antibody BCL2-like 10 (apoptosis faci |
|---|---|
| Target | BCL2L10 |
| Molecular Weight(MW) | 22 kDa (note) (2) |
| Cellular Localization | Mitochondrion , Nucleus membrane |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using BCL2L10 antibody at 1:200 dilution.
Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL2L10 antibody [N2C2], Internal diluted at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using BCL2L10 antibody at 1:100 dilution.| Positive Control | Jurkat , Raji , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
