Application
Immunohistochemical analysis of paraffin-embedded human tonsil, using BCL6 antibody(10 μg/ml).Application
BCL6 antibody [N2C1], Internal detects BCL6 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE BCL6 antibody [N2C1], Internal dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: NCI-H929 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 μg whole cell lysate/extract of BCL6 protein expressing Raji cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 μg of BCL6 antibody 7.5% SDS-PAGE The immunoprecipitated BCL6 protein was detected by BCL6 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.| Product Name | BCL6 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCL6 (B-cell CLL/lymphoma 6)The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BCL6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Rhesus Monkey, BovineRat (92%), Dog (90%), Pig (89%), Rhesus Monkey (97%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BCL5 antibody BCL6A antibody LAZ3 antibody ZBTB27 antibody ZNF51 antibody BCL6 antibody B-cell lymphoma 6 protein antibody lymphoma-associated zinc finger gene on chromosome 3 antibody zinc finger transcription factor BCL6S antibody B-cell lymphom |
|---|---|
| Molecular Weight(MW) | 79 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded human tonsil, using BCL6 antibody(10 μg/ml).
Application
BCL6 antibody [N2C1], Internal detects BCL6 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE BCL6 antibody [N2C1], Internal dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: NCI-H929 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 μg whole cell lysate/extract of BCL6 protein expressing Raji cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 μg of BCL6 antibody 7.5% SDS-PAGE The immunoprecipitated BCL6 protein was detected by BCL6 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.| Positive Control | Jurkat , Raji , NCI-H929 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
