Application
Sample(30 μg of whole cell lysate) A:HeLa S3 15% SDS PAGE diluted at 1:1000Application
BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCL7A protein stained by BCL7A antibody [C2C3], C-term diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded oral CA cell lines Ca922 xenograft, using BCL7A antibody at 1:100 dilution.Application
BCL7A antibody [C2C3], C-term detects BCL7A protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract 12 % SDS-PAGE BCL7A antibody [C2C3], C-term dilution: 1:1000Application
BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. BCL7A antibody [C2C3], C-term dilution: 1:500.Application
BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. BCL7A antibody [C2C3], C-term dilution: 1:500.Application
BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. BCL7A antibody [C2C3], C-term dilution: 1:500.| Product Name | BCL7A antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCL7A (B-cell CLL/lymphoma 7A)This gene is directly involved, with Myc and IgH, in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene product is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BCL7A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BCL7 antibody BCL7A antibody B-cell CLL/lymphoma 7 protein family member A antibody B-cell CLL/lymphoma-7 antibody B-cell CLL/lymphoma 7A antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa (note) (2) |
Application
Sample(30 μg of whole cell lysate) A:HeLa S3 15% SDS PAGE diluted at 1:1000
Application
BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCL7A protein stained by BCL7A antibody [C2C3], C-term diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded oral CA cell lines Ca922 xenograft, using BCL7A antibody at 1:100 dilution.
Application
BCL7A antibody [C2C3], C-term detects BCL7A protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract 12 % SDS-PAGE BCL7A antibody [C2C3], C-term dilution: 1:1000
Application
BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. BCL7A antibody [C2C3], C-term dilution: 1:500.
Application
BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. BCL7A antibody [C2C3], C-term dilution: 1:500.
Application
BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. BCL7A antibody [C2C3], C-term dilution: 1:500.| Positive Control | HeLaS3 , Neuro2A , GL261 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
