Application
ICC/IF staining of methanol-fixed HeLa cells using Bcl-X (phospho Ser62) antibody (red).Application
WB analysis of extracts from untreated and EGF-treated HeLa cells using Bcl-X (phospho Ser62) antibody.Application
IHC-P analysis of human breast carcinoma tissue using Bcl-X (phospho Ser62) antibody.| Product Name | Bcl-X (phospho Ser62) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Bcl-X (phospho Ser62)The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008] |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S62 of human BCL2L1 |
| Modification | p-Ser62 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:100. IF: 1:50 - 1:100.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCLXS BCL2L BCL-XL/S bcl-xL BCLX bcl-xS BCL2L1 PPP1R52 Bcl-X BCL2-like 1 BCLXL |
|---|---|
| Target | Bcl-X (phospho Ser62) |
| Molecular Weight(MW) | 26 kDa (note) |
Application
ICC/IF staining of methanol-fixed HeLa cells using Bcl-X (phospho Ser62) antibody (red).
Application
WB analysis of extracts from untreated and EGF-treated HeLa cells using Bcl-X (phospho Ser62) antibody.
Application
IHC-P analysis of human breast carcinoma tissue using Bcl-X (phospho Ser62) antibody.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:100. IF: 1:50 - 1:100.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
