Application
ApplicationApplication
WB analysis of human lung cell extract using Bcl-X antibody [2H12].| Product Name | Bcl-X antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to bcl - X ( Apoptosis Marker )This antibody recognizes a 27 kD protein identified as bcl-X protein. This antibody is highly specific to bcl-X and does not show cross reactivity with bcl-2 alpha or bax protein. This antibody cross reacts with human, mouse, rat and pig. |
| Immunogen | BALB/C mice were injected with a synthetic peptide of amino acid 3-14 (Cys-QSNRELVVDFLS) of human bcl-X protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2H12 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Can also be used for: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100): |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp781P2092 BCL-XL/S BCL2L1 bclxL BclX 600039 BCLXL/S 598 Q07817 bcl-xL Bcl-X bclxS BCL2L bcl-xS Bcl X bcl xL bcl xS BCL XL/S |
|---|---|
| Target | Bcl-X |
| Cellular Localization | Cytoplasmic, cell membrane |
Application
Application
Application
WB analysis of human lung cell extract using Bcl-X antibody [2H12].| Positive Control | Hodgkin’s lymphoma |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Can also be used for: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100): |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
