Application
Formalin-fixed, paraffin-embedded human Hodgkins lymphoma stained with using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of Reed-Sternberg cells.| Product Name | Bcl-X antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2H12] to Bcl xBcl-X has two isoforms, Bcl-X L (long) a 241 amino acid protein and Bcl-X S (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 family. |
| Immunogen | A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein. This amino acid sequence is shared by human and murine bcl-X protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2H12 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-P |
| Flow Cytometry / Immunofluorescence: assay dependent. Immunoprecipitation (native): use at a concentration of 2μg / mg. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4μg / ml for 30 min at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.: |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | DKFZp781P2092 BCL-XL/S BCL2L1 bclxL BclX 600039 BCLXL/S 598 Q07817 bcl-xL Bcl-X bclxS BCL2L bcl-xS Bcl X bcl xL bcl xS BCL XL/S |
|---|---|
| Target | Bcl-X |
| Tissue Specificity | reacts with both Bcl-XS and Bcl-XL proteins. |
| Cellular Localization | Mitochondrial membranes and perinuclear envelope |
Application
Formalin-fixed, paraffin-embedded human Hodgkins lymphoma stained with using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of Reed-Sternberg cells.| Positive Control | Hodgkin's lymphoma |
|---|---|
| Application Notes | Flow Cytometry / Immunofluorescence: assay dependent. Immunoprecipitation (native): use at a concentration of 2μg / mg. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4μg / ml for 30 min at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
