Application
Flow cytometric Analysis of Hela cells, using anti-BDH2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-BDH2 mouse monoclonal antibody. ( Dilution 1:50)Application
Anti-BDH2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BDH2Application
HEK293T cells transfected with either RC210586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BDH2 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BDH2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BDH2.Application
Flow cytometric Analysis of Jurkat cells, using anti-BDH2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Product Name | BDH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4G4] to BDH2Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase. |
| Immunogen | Full length human recombinant protein of human BDH2(NP_064524) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 4G4 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ13261 UCPAOR PRO20933 BDH2 UCPA-OR UNQ6308 UCPA OR |
|---|---|
| Target | BDH2 |
| Tissue Specificity | is specific for human BDH2(NP_064524). |
Application
Flow cytometric Analysis of Hela cells, using anti-BDH2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-BDH2 mouse monoclonal antibody. ( Dilution 1:50)
Application
Anti-BDH2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BDH2
Application
HEK293T cells transfected with either RC210586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BDH2 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BDH2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BDH2.
Application
Flow cytometric Analysis of Jurkat cells, using anti-BDH2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
