Application
Western Blot: Beta Actin Antibody [ - Detection of actin in 3T3 (20ug) lysates ECL detection 30 seconds. Lane 1 - 1:15,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - 1:15,000 dilutionApplication
Western Blot: Beta Actin Antibody [ - Detection of Beta Actin. Lane 1: 5ug, nuclear extract from mouse bone marrow derived macrophages. Lane 2: 10ug, cytoplasmic extract from mouse bone narrow derived macrophages. Imageprovided via product review by verified customer.Application
Western Blot: Beta Actin Antibody [ - Western blot analysis of Actin using HepG2 whole cell lysateApplication
Western Blot: Beta Actin Antibody [ - Western blot analysis of Actin using RCC4 whole cell lysate.| Product Name | beta Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to beta ActinThis gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to the N-terminus of human Beta Actin (Swiss-Prot entry P60709). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:5,000-1:15,000. A 40 kDa band is detected in HeLa whole cell lysate and mouse NIH3T3 cells. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PS1TP5BP1 , BRWS1 , ACTB , actin, beta |
|---|---|
| Target | beta Actin |
| Molecular Weight(MW) | 40 kDa (note) |
Application
Western Blot: Beta Actin Antibody [ - Detection of actin in 3T3 (20ug) lysates ECL detection 30 seconds. Lane 1 - 1:15,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - 1:15,000 dilution
Application
Western Blot: Beta Actin Antibody [ - Detection of Beta Actin. Lane 1: 5ug, nuclear extract from mouse bone marrow derived macrophages. Lane 2: 10ug, cytoplasmic extract from mouse bone narrow derived macrophages. Imageprovided via product review by verified customer.
Application
Western Blot: Beta Actin Antibody [ - Western blot analysis of Actin using HepG2 whole cell lysate
Application
Western Blot: Beta Actin Antibody [ - Western blot analysis of Actin using RCC4 whole cell lysate.| Positive Control | HeLa Whole Cell Lysate |
|---|---|
| Application Notes | WB: 1:5,000-1:15,000. A 40 kDa band is detected in HeLa whole cell lysate and mouse NIH3T3 cells. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C. Do not freeze. |
| Storage Buffer | Tris-citrate/phosphate containing 0.09% sodium azide, pH 7-8. |
