Application
beta Actin antibody detects beta Actin protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
beta Actin antibody detects beta Actin protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10% SDS-PAGE beta Actin antibody dilution: 1:20000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of beta Actin protein from 293T whole cell extracts using 5 μg of beta Actin antibody . Western blot analysis was performed using beta Actin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:10000.Application
beta Actin antibody detects beta Actin protein at cytoplasm in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. beta Actin antibody diluted at 1:500.Application
beta Actin antibody detects ACTB protein by western blot analysis. A. 30 µg drosophila lysate/extract 10% SDS-PAGE beta Actin antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
beta Actin antibody detects beta Actin protein at cytoplasm and cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: beta Actin protein stained by beta Actin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. beta Actin antibody diluted at 1:500.Application
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. beta Actin antibody diluted at 1:500.Application
beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
beta Actin antibody detects beta Actin protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | beta Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Actin (actin, beta)This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIHC-RIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | DrosophilaHumanMouseRat |
| Predicted Reactivity | Japanese medaka, Xenopus laevis, Dog, Rabbit, Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine, Guinea pigJapanese medaka (98%), Xenopus laevis (99%), Dog (100%), Rabbit (99%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PS1TP5BP1 antibody, ACTB antibody, |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
beta Actin antibody detects beta Actin protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
beta Actin antibody detects beta Actin protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10% SDS-PAGE beta Actin antibody dilution: 1:20000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of beta Actin protein from 293T whole cell extracts using 5 μg of beta Actin antibody . Western blot analysis was performed using beta Actin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at 1:10000.
Application
beta Actin antibody detects beta Actin protein at cytoplasm in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. beta Actin antibody diluted at 1:500.
Application
beta Actin antibody detects ACTB protein by western blot analysis. A. 30 µg drosophila lysate/extract 10% SDS-PAGE beta Actin antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
beta Actin antibody detects beta Actin protein at cytoplasm and cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: beta Actin protein stained by beta Actin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. beta Actin antibody diluted at 1:500.
Application
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. beta Actin antibody diluted at 1:500.
Application
beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
beta Actin antibody detects beta Actin protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , mouse liver , PC-12 , Rat2 , rat liver , drosophila |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
