Application
Western blot of A431 cells, using anti-β-Actin Clone RM112 at a 1/1000 dilution, showed a band of β-Actin at the predicted MW (42 kDa).Application
Immunocytochemical staining of HeLa cells, using anti-β-Actin Clone RM112 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).| Product Name | beta actin antibody [RM112] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM112] to beta actinThis gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008] |
| Immunogen | A peptide corresponding to the N-terminus of β-actin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM112 |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHCIPWB |
| Western Blot (WB): 1:1000 dilution, Immunoprecipitation (IP): 1:200 dilution, Chromatin IP (ChIP): 1:200 dilution, Immunocytochemistry (ICC): 1:200 dilution, Immunohistochemistry (IHC): 1:200 dilution. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PS1TP5BP1 , BRWS1 , actin, beta , ACTB |
|---|---|
| Target | beta actin |
| Tissue Specificity | This antibody reacts to β-Actin (Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin. |
Application
Western blot of A431 cells, using anti-β-Actin Clone RM112 at a 1/1000 dilution, showed a band of β-Actin at the predicted MW (42 kDa).
Application
Immunocytochemical staining of HeLa cells, using anti-β-Actin Clone RM112 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).| Application Notes | Western Blot (WB): 1:1000 dilution, Immunoprecipitation (IP): 1:200 dilution, Chromatin IP (ChIP): 1:200 dilution, Immunocytochemistry (ICC): 1:200 dilution, Immunohistochemistry (IHC): 1:200 dilution. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
