Application
Western blot analysis of extracts from MCF-7 cells untreated or treated with Calyculin A using b-Catenin(Phospho-Ser33) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Phospho-Ser33) Antibody (left) or the same antibody preincubated with blocking peptide(right)| Product Name | beta Catenin (phospho Ser33) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Catenin (Phospho Ser33)Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway. |
| Immunogen | Peptide sequence around phosphorylation site of serine 33 (L-D-S(p)-G-I) derived from Human beta -Catenin. |
| Modification | p-Ser33 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CTNNB, DKFZp686D02253, FLJ25606, FLJ37923, CTNNB1, catenin beta-1, catenin (cadherin-associated protein), beta 1, 88kDa |
|---|---|
| Target | beta Catenin (Phospho Ser33) |
| Molecular Weight(MW) | 92 (note) |
Application
Western blot analysis of extracts from MCF-7 cells untreated or treated with Calyculin A using b-Catenin(Phospho-Ser33) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Phospho-Ser33) Antibody (left) or the same antibody preincubated with blocking peptide(right)| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
