Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin (phospho Ser33/Ser37/Thr41) antibody diluted at 1:500.| Product Name | beta Catenin (phospho Ser33/Ser37/Thr41) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Catenin (phospho Ser33/Ser37/Thr41) (catenin (cadherin-associated protein), beta 1, 88kDa)Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human beta Catenin (phospho Ser33/Ser37/Thr41). The exact sequence is proprietary. |
| Modification | p-Ser33/Ser37/Thr41 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Dog, Pig, Chicken, Sheep, Rhesus Monkey, BovineMouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | beta Catenin |
|---|---|
| Molecular Weight(MW) | 85 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Nucleus , cytoskeleton |
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin (phospho Ser33/Ser37/Thr41) antibody diluted at 1:500.| Positive Control | HeLa (100 nM Calyculin A treatment for 0.5 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
