Application
WB ImageWB analysis of beta Galactosidase protein (lanes1-3 for 60 ng, 30 ng and 15 ng, respectively) and non-specific control E.coli lysate (lane 4) using beta Galactosidase antibody.Application
WB ImageWB analysis of beta Galactosidase protein (1 μg) using beta Galactosidase antibody at a dilution of 1:1,000.Application
WB ImageWB analysis of non-reduced and reduced beta Galactosidase protein (lane1 for 80 ng and lane 2 for 20 ng) using beta Galactosidase antibody at a dilution of 1:10,000.| Product Name | beta Galactosidase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to beta GalactosidaseBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used. |
| Immunogen | Beta-Galactosidase (E.coli) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ConjugationDotELISAICC/IFIHC-FIHC-PIPWB |
| For ELISA: Use at a dilution of 1:4,000 - 1:16,000. For WB: Use at a dilution of 1:5000 - 1:10000. For IHC-Fr and IHC-P: Use at a dilution of 1:500 - 1:3,000. For all other applications: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | E.coli |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Unpurified |
| Gene Synonyms | Beta Galactosidase [E.coli], BGAL, beta-galactosidase, beta-gal |
|---|---|
| Target | beta Galactosidase |
| Tissue Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta-Galactosidase (E.coli). |
Application
WB ImageWB analysis of beta Galactosidase protein (lanes1-3 for 60 ng, 30 ng and 15 ng, respectively) and non-specific control E.coli lysate (lane 4) using beta Galactosidase antibody.
Application
WB ImageWB analysis of beta Galactosidase protein (1 μg) using beta Galactosidase antibody at a dilution of 1:1,000.
Application
WB ImageWB analysis of non-reduced and reduced beta Galactosidase protein (lane1 for 80 ng and lane 2 for 20 ng) using beta Galactosidase antibody at a dilution of 1:10,000.| Application Notes | For ELISA: Use at a dilution of 1:4,000 - 1:16,000. For WB: Use at a dilution of 1:5000 - 1:10000. For IHC-Fr and IHC-P: Use at a dilution of 1:500 - 1:3,000. For all other applications: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% sodium azide |
