Application
ELISA detection of Beta-lactoglobulin using for detection at a concentration of 0.2 µg/mL.Application
beta Lactoglobulin antibody detects Beta lactoglobulin protein by western blot analysis. Non-fat milk (0.1 µg and 0.05 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta Lactoglobulin antibody diluted by 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | beta Lactoglobulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Affinity Purified Rabbit anti-Bovine beta LactoglobulinPrimary component of whey, it binds retinol and is probably involved in the transport of that molecule. |
| Immunogen | Rabbits were immunized with bovine beta lactoglobulin. After multiple immunization in Freund's adjuvant the rabbits were bled, serum collected and the antibodies isolated by immunoaffinity chromatography |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIEWBFunctional Assay |
| Suggested starting dilutions are as follows: WB: 1:5000-1:20000, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Bovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LGB antibody, MGC134239 antibody |
|---|---|
| Target | beta Lactoglobulin |
Application
ELISA detection of Beta-lactoglobulin using for detection at a concentration of 0.2 µg/mL.
Application
beta Lactoglobulin antibody detects Beta lactoglobulin protein by western blot analysis. Non-fat milk (0.1 µg and 0.05 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta Lactoglobulin antibody diluted by 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | non-fat milk |
|---|---|
| Application Notes | Suggested starting dilutions are as follows: WB: 1:5000-1:20000, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS. 0.1% Sodium Azide was added as a preservative. |
