Rabbit polyclonal antibody to beta III TubulinTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Immunogen
Peptide sequence around aa.442~446(E-E-S-E-A) derived from Human Tubulin beta-III.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Species Reactivity
HumanMouse
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
CFEOM3A, TUBB4, beta-4, TUBB3, tubulin beta-3 chain, class III beta-tubulin, tubulin beta-III, tubulin beta-4 chain, tubulin, beta 3
Target
beta III Tubulin
Molecular Weight(MW)
55 (note)
Application
Application
Western Blot analysis of extract from Mouse brain tissue,Hela,JK and P19 cells using Tubulin beta-III Antibody.
Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.