Application
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI .Application
Various tissue extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody diluted at 1:10000.Application
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody diluted at a dilution of 1:10000.Application
beta Tubulin 3/ Tuj1 antibody detects beta Tubulin 3/ Tuj1 protein at cytoskeleton by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | beta Tubulin 3/ Tuj1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Tubulin 3/ TUJ1 (tubulin, beta 3 class III)This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Tubulin 3/ TUJ1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chimpanzee, BovineChimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CFEOM3A , beta-4 , CDCBM , tubulin, beta 3 class III , TUBB4 , TUBB3 |
|---|---|
| Target | beta Tubulin 3/ TUJ1 |
| Molecular Weight(MW) | 50 kDa (note) (2) |
Application
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
Various tissue extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody diluted at 1:10000.
Application
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody diluted at a dilution of 1:10000.
Application
beta Tubulin 3/ Tuj1 antibody detects beta Tubulin 3/ Tuj1 protein at cytoskeleton by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HeLa , SK-N-SH , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
