Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody diluted at 1:50000.Application
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody diluted at a dilution of 1:1000.Application
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. beta Tubulin 3/ TUJ1 antibody diluted at 1:500.Application
Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Application
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI .Application
Immunoprecipitation of beta Tubulin 3/ TUJ1 protein from SK-N-SH whole cell extracts using 5 μg of beta Tubulin 3/ TUJ1 antibody . Western blot analysis was performed using beta Tubulin 3/ TUJ1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | beta Tubulin 3/ Tuj1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Tubulin 3/ TUJ1 (tubulin, beta 3 class III)This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human beta Tubulin 3/ TUJ1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TUBB4 , TUBB3 , CFEOM3A , tubulin, beta 3 class III , CDCBM , beta-4 |
|---|---|
| Target | beta Tubulin 3/ TUJ1 |
| Molecular Weight(MW) | 50 kDa (note) |
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody diluted at 1:50000.
Application
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody diluted at a dilution of 1:1000.
Application
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. beta Tubulin 3/ TUJ1 antibody diluted at 1:500.
Application
Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Application
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
Immunoprecipitation of beta Tubulin 3/ TUJ1 protein from SK-N-SH whole cell extracts using 5 μg of beta Tubulin 3/ TUJ1 antibody . Western blot analysis was performed using beta Tubulin 3/ TUJ1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
