Application
beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein at cytosol on Mahlarvu xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Mahlarvu xenograft. beta Tubulin 8 antibody [C2C3], C-term dilution: 1:500.Application
beta Tubulin 8 antibody immunoprecipitates beta Tubulin 8 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of beta Tubulin 8 protein by 2 μg of beta Tubulin 8 antibody 10% SDS-PAGE The immunoprecipitated beta Tubulin 8 protein was detected by beta Tubulin 8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
beta Tubulin 8 antibody [C2C3], C-term detects beta Tubulin 8 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 8 protein stained by beta Tubulin 8 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using beta Tubulin 8 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample(30 ug whole cell lysate) A:A431 B:H1299 10% SDS PAGE diluted at 1:1000Application
beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 10 % SDS-PAGE beta Tubulin 8 antibody [C2C3], C-term dilution: 1:1000| Product Name | beta Tubulin 8 antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Tubulin 8Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ40100 antibody, TUBB8 antibody, tubulin beta-8 chain antibody, HSA10p15 beta-tubulin 4Q antibody, class VIII beta-tubulin antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (2) |
Application
beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein at cytosol on Mahlarvu xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Mahlarvu xenograft. beta Tubulin 8 antibody [C2C3], C-term dilution: 1:500.
Application
beta Tubulin 8 antibody immunoprecipitates beta Tubulin 8 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of beta Tubulin 8 protein by 2 μg of beta Tubulin 8 antibody 10% SDS-PAGE The immunoprecipitated beta Tubulin 8 protein was detected by beta Tubulin 8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
beta Tubulin 8 antibody [C2C3], C-term detects beta Tubulin 8 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 8 protein stained by beta Tubulin 8 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using beta Tubulin 8 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample(30 ug whole cell lysate) A:A431 B:H1299 10% SDS PAGE diluted at 1:1000
Application
beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 10 % SDS-PAGE beta Tubulin 8 antibody [C2C3], C-term dilution: 1:1000| Positive Control | A431 , H1299 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
