Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded AGS xenograft, using beta Tubulin antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spinal cord by immunohistochemical analysis. Sample: Paraffin-embedded rat spinal cord. beta Tubulin antibody diluted at 1:500.Application
beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. beta Tubulin antibody diluted at 1:500.Application
beta Tubulin antibody detects beta Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody diluted at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
beta Tubulin antibody detects beta Tubulin protein at cytoplasm on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. beta Tubulin antibody dilution: 1:500.| Product Name | beta Tubulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to beta Tubulin (tubulin, beta) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRatHamster |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineXenopus laevis (99%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%),Fruit flies |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | M40 antibody, MGC117247 antibody, MGC16435 antibody, OK/SW-cl.56 antibody, TUBB1 antibody, TUBB5 antibody, TUBB antibody, beta1-tubulin antibody, beta-4 tubulin antibody, tubulin beta-1 chain antibody, tubulin beta chain antibody, tubulin beta polypeptide |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (5) |
Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded AGS xenograft, using beta Tubulin antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spinal cord by immunohistochemical analysis. Sample: Paraffin-embedded rat spinal cord. beta Tubulin antibody diluted at 1:500.
Application
beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. beta Tubulin antibody diluted at 1:500.
Application
beta Tubulin antibody detects beta Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody diluted at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
beta Tubulin antibody detects beta Tubulin protein at cytoplasm on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. beta Tubulin antibody dilution: 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 , *MCF7 , *Huh7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
