Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000Application
Sample(30 μg of whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:500| Product Name | beta-Adaptin antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to beta-Adaptin (adaptor-related protein complex 1, beta 1 subunit)Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Two transcript variants encoding different isoforms have been found for this gene, and variants utilizing alternative polyadenylation signals exist. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human beta-Adaptin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, BovineRat (98%), Zebrafish (99%), Xenopus laevis (99%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ADTB1 antibody, AP105A antibody, BAM22 antibody, CLAPB2 antibody, AP1B1 antibody, adapter-related protein complex 1 subunit beta-1 antibody, Golgi adaptor HA1/AP1 adaptin beta subunit antibody, AP-1 complex subunit beta-1 antibody, adaptor protein complex |
|---|---|
| Target | beta-Adaptin |
| Molecular Weight(MW) | 105 kDa (note) (3) |
| Cellular Localization | Golgi apparatus , Cytoplasmic vesicle , clathrin-coated vesicle membrane |
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000
Application
Sample(30 μg of whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:500| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
