Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000Application
beta-TrCP antibody [N1C1] detects beta-TrCP protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. beta-TrCP antibody [N1C1] dilution: 1:250.| Product Name | beta-TrCP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to beta-TrCP (beta-transducin repeat containing)This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human beta-TrCP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, BovineMouse (99%), Xenopus laevis (96%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BETA-TRCP antibody, FBW1A antibody, FBXW1 antibody, FBXW1A antibody, FWD1 antibody, MGC4643 antibody, bTrCP antibody, bTrCP1 antibody, betaTrCP antibody, BTRC antibody, E3RSIkappaB antibody, F-box/WD repeat-containing protein 1A antibody, F-box and WD repe |
|---|---|
| Target | beta-TrCP |
| Molecular Weight(MW) | 69 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000
Application
beta-TrCP antibody [N1C1] detects beta-TrCP protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. beta-TrCP antibody [N1C1] dilution: 1:250.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
