Application
Immunofluorescence analysis of methanol-fixed A431, using BHMT antibody at 1:500 dilution.Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BHMT antibody diluted at 1:1000.Application
Sample(30 ug whole cell lysate) A:Hep G2 10% SDS PAGE diluted at 1:1000Application
BHMT antibody detects BHMT protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. BHMT antibody dilution: 1:500.| Product Name | BHMT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BHMT (betaine--homocysteine S-methyltransferase)This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BHMT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, BovineRat (93%), Zebrafish (82%), Xenopus laevis (87%), Pig (95%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BHMT1 antibody, BHMT antibody, betaine--homocysteine S-methyltransferase 1 antibody, betaine--homocysteine S-methyltransferase antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed A431, using BHMT antibody at 1:500 dilution.
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BHMT antibody diluted at 1:1000.
Application
Sample(30 ug whole cell lysate) A:Hep G2 10% SDS PAGE diluted at 1:1000
Application
BHMT antibody detects BHMT protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. BHMT antibody dilution: 1:500.| Positive Control | HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
