Application
Anti-BHMT mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BHMTApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT .Application
Flow cytometric Analysis of Jurkat cells, using anti-BHMT antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Flow cytometric Analysis of Hela cells, using anti-BHMT antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Product Name | BHMT antibody [10B3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [10B3] to BHMTInvolved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. |
| Immunogen | Full length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 10B3 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BHMT1 |
|---|---|
| Target | BHMT |
| Tissue Specificity | is specific for human BHMT (NP_001704). |
Application
Anti-BHMT mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BHMT
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT .
Application
Flow cytometric Analysis of Jurkat cells, using anti-BHMT antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Flow cytometric Analysis of Hela cells, using anti-BHMT antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
