Application
Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:20000Application
Immunofluorescence analysis of methanol-fixed HeLa, using BHMT2 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE diluted at 1:1000| Product Name | BHMT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BHMT2 (betaine-homocysteine methyltransferase 2)Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. The protein encoded by this gene is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BHMT2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, PigRat (89%), Pig (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ20001 antibody, BHMT2 antibody, betaine-homocysteine methyltransferase 2 antibody, betaine--homocysteine S-methyltransferase 2 antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
Application
Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:20000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using BHMT2 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE diluted at 1:1000| Positive Control | HCT116 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
