Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:1000Application
Sample(30 μg of whole cell lysate) A:H1299 7.5% SDS PAGE diluted at 1:1000Application
Bif-1 antibody [C2C3], C-term detects Bif-1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: Bif-1 protein stained by Bif-1 antibody [C2C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μmApplication
Bif-1 antibody detects Bif-1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bif-1 antibody dilution: 1:500.| Product Name | Bif-1 antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Bif1 (SH3-domain GRB2-like endophilin B1)May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Bif-1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, Rhesus Monkey, ChimpanzeeRat (100%), Xenopus laevis (100%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bif-1 antibody, KIAA0491 antibody, dJ612B15.2 antibody, SH3GLB1 antibody, Bax-interacting factor 1 antibody, endophilin-B1 antibody, SH3 domain-containing GRB2-like protein B1 antibody, protein phosphatase 1, regulatory subunit 70 antibody, SH3-containing |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein (By similarity) , Mitochondrion outer membrane; Peripheral membrane protein |
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:1000
Application
Sample(30 μg of whole cell lysate) A:H1299 7.5% SDS PAGE diluted at 1:1000
Application
Bif-1 antibody [C2C3], C-term detects Bif-1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: Bif-1 protein stained by Bif-1 antibody [C2C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm
Application
Bif-1 antibody detects Bif-1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bif-1 antibody dilution: 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
