Application
Western blot analysis of extracts from K562 cells, using BIK (Phospho-Thr33) antibody. The lane on the right is treated with the synthesized peptide.Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using BIK (Phospho-Thr33) antibody. The picture on the right is treated with the synthesized peptide.| Product Name | BIK (phospho Thr33) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BIK (phospho Thr33)The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq, Sep 2011] |
| Immunogen | Peptide sequence around phosphorylation site of threonine 33 (G-M-T(p)-D-S) derived from Human BIK. |
| Modification | p-Thr33 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:3000. IHC: 1:50 - 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NBK , BIK , BIP1 , BCL2-interacting killer (apoptosis-inducing) , BP4 |
|---|---|
| Target | BIK (phospho Thr33) |
| Tissue Specificity | The antibody detects endogenous levels of BIK only when phosphorylated at threonine 33. |
Application
Western blot analysis of extracts from K562 cells, using BIK (Phospho-Thr33) antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using BIK (Phospho-Thr33) antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | WB: 1:500 - 1:3000. IHC: 1:50 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
