Application
Western blot analysis of BIK expression in Jurkat (Lane 1), mouse intestine (Lane 2) and rat kidney (Lane 3) lysates.| Product Name | BIK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BIKBIK is a member of Pro-apoptotic Bcl-2 family proteins. Although Bik lacks Bcl-2 homologus region, BH1 and BH2, BIK interacts with Bcl-2 and Bcl-xL. BIK has been shown to induce apoptosis by activating caspase-9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (2-6 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate, mouse small intestine and rat kidney can be used as positive controls. The antibody detects a ~17 kDa BIK in samples from human, mouse, and rat origins. A ~15 kDa cleavage fragment can also be detected | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BIP, BIK, BIP1, BIP 1, BCL2 interacting killer, Apoptosis inducer NBK |
|---|---|
| Target | BIK |
| Tissue Specificity | This antibody is specific for BIK. |
| Cellular Localization | Around the nuclear envelope, and in cytoplasmic membranes. |
Application
Western blot analysis of BIK expression in Jurkat (Lane 1), mouse intestine (Lane 2) and rat kidney (Lane 3) lysates.| Application Notes | Western blotting (2-6 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate, mouse small intestine and rat kidney can be used as positive controls. The antibody detects a ~17 kDa BIK in samples from human, mouse, and rat origins. A ~15 kDa cleavage fragment can also be detected |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
