Application
Immunofluorescence of Bim in K562 cells with Bim antibody at 20 ug/mL.Application
Western blot analysis of Bim in K562 cell lysates with Bim antibody at (A) 2.5 and (B) 5 ug/mL.Application
Immunocyochemistry of BIM in K562 cells with Bim antibody at 10 ug/mL.| Product Name | Bim antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BimThe protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | Bim antibody was raised against a peptide corresponding to amino acids near the center of human Bim. The sequence is identical to that of mouse and rat. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB: 2.5 - 5 ug/ml. ICC: 10 ug/ml. IF: 20 ug/ml | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BCL2-like 11 (apoptosis facilitator) , BIM , BOD , BCL2L11 , BAM |
|---|---|
| Target | Bim |
Application
Immunofluorescence of Bim in K562 cells with Bim antibody at 20 ug/mL.
Application
Western blot analysis of Bim in K562 cell lysates with Bim antibody at (A) 2.5 and (B) 5 ug/mL.
Application
Immunocyochemistry of BIM in K562 cells with Bim antibody at 10 ug/mL.| Application Notes | WB: 2.5 - 5 ug/ml. ICC: 10 ug/ml. IF: 20 ug/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
