Application
Western blot analysis of extracts of SW620 cell line, using BIN1 antibody.Application
Immunofluorescence analysis of U2OS cell using BIN1 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded Rat brain using BIN1 antibody at dilution of 1:100 (x400 lens).Application
Immunohistochemistry of paraffin-embedded Human kidney using BIN1 antibody at dilution of 1:100 (x400 lens).| Product Name | BIN1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BIN1This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq, Sep 2011] |
| Immunogen | Recombinant protein of human BIN1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:20 - 1:50 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | bridging integrator 1 , AMPHL , SH3P9 , AMPH2 |
|---|---|
| Target | BIN1 |
Application
Western blot analysis of extracts of SW620 cell line, using BIN1 antibody.
Application
Immunofluorescence analysis of U2OS cell using BIN1 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded Rat brain using BIN1 antibody at dilution of 1:100 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded Human kidney using BIN1 antibody at dilution of 1:100 (x400 lens).| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:20 - 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
