Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using BIN1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using BIN1 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Molt-4 B: Raji 10% SDS PAGE diluted at 1:1000| Product Name | BIN1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BIN1 (bridging integrator 1)This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BIN1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Chicken, BovineMouse (93%), Rat (92%), Xenopus laevis (81%), Pig (94%), Chicken (84%), Bovine (93%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AMPH2 antibody, AMPHL antibody, DKFZp547F068 antibody, MGC10367 antibody, SH3P9 antibody, BIN1 antibody, box-dependent myc-interacting protein 1 antibody, amphiphysin-like protein antibody, amphiphysin II antibody, box dependant MYC interacting protein 1 a |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) (9) |
| Cellular Localization | Isoform BIN1: Nucleus , Isoform IIA: Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using BIN1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using BIN1 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Molt-4 B: Raji 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
