Application
Immunofluorescence of Bit1 in 3T3 cells with Bit1 antibody at 20 ug/mL.Application
Western blot analysis of Bit1 in A-20 cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.Application
Immunocytochemistry of Bit1 in 3T3 cells with Bit1 antibody at 2 ug/ml.| Product Name | Bit1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Bit1Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2) |
| Immunogen | Human Bit1 / Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2 (N-Terminus) Peptide |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bit1, Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2, Bcl2 inhibitor of transcription1 / PeptidyltRNA hydrolase2, Bcl2 inhibitor of transcription 1 / PeptidyltRNA hydrolase 2 |
|---|---|
| Target | Bit1 |
| Tissue Specificity | This antibody is specific for Bit1 N-Terminus |
Application
Immunofluorescence of Bit1 in 3T3 cells with Bit1 antibody at 20 ug/mL.
Application
Western blot analysis of Bit1 in A-20 cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
Application
Immunocytochemistry of Bit1 in 3T3 cells with Bit1 antibody at 2 ug/ml.| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
