Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue using BLNK (Phospho-Tyr84) antibodyApplication
Western blot analysis of extracts from K562 cells, treated and starved (24 hrs), using BLNK (Phospho-Tyr84) antibody| Product Name | BLNK (phospho Tyr84) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BLNK ( phospho Y84 )The B cell linker protein BLNK (also referred to as B cell adaptor containing Src homology 2 domain - BASH - or, SLP-65), is crucial for B cell antigen receptor (BCR)-mediated activation, proliferation, and differentiation of B cells. BLNK represents a central linker protein that bridges the BCR-associated kinases with a multitude of signaling pathways and may regulate the biological responses of B cell function and development. Tyrosine phosphorylation of BLNK by Syk provides docking sites for SH2-containing effector molecules such as PLC alpha, Btk, Vav, Grb2 and Nck, and contributes to the activation of the IKK complex and the resulting NF-kappaB signaling pathway. |
| Immunogen | Synthetic phospho peptide (Human) derived from a region of BLNK that contains tyrosine 84. The sequence is conserved in mouse. |
| Modification | p-Tyr84 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: Use at a concentration of 0.1 - 1 μg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 54.7 kDa). Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 604515, MGC111051, SLP65, Ly57, 29760, SLP-65, BASH, BLNK-s, BLNKs, BLNK, Q8WV28, SLP 65, BLNK s, BLNK (phospho Y84), BLNK (phospho Tyr84), BLNK (pY84), BLNK (pTyr84), BLNK phospho Y84, BLNK phospho Tyr84, Phospho BLNK (Y84), Phospho BLNK (Tyr84), Phospho |
|---|---|
| Target | BLNK |
Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue using BLNK (Phospho-Tyr84) antibody
Application
Western blot analysis of extracts from K562 cells, treated and starved (24 hrs), using BLNK (Phospho-Tyr84) antibody| Application Notes | WB: Use at a concentration of 0.1 - 1 μg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 54.7 kDa). Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA. pH 7.3 |
