Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue using BLNK (Phospho-Tyr84) antibody. The picture on the right is treated with the synthesized peptide.Application
Western blot analysis of extracts from K562 cells, treated with starved (24hours), using BLNK (Phospho-Tyr84) antibody. The lane on the right is treated with the synthesized peptide.| Product Name | BLNK (phospho Tyr84) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BLNK (phospho Tyr84)This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012] |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 84 (E-M-Y(p)-V-M) derived from Human BLNK. |
| Modification | p-Tyr84 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:3000. IHC: 1:50 - 1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | B-cell linker , BLNK , AGM4 , SLP65 , SLP-65 , LY57 , bca , BLNK-S , BASH |
|---|---|
| Target | BLNK (phospho Tyr84) |
| Tissue Specificity | The antibody detects endogenous levels of BLNK only when phosphorylated at tyrosine 84. |
Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue using BLNK (Phospho-Tyr84) antibody. The picture on the right is treated with the synthesized peptide.
Application
Western blot analysis of extracts from K562 cells, treated with starved (24hours), using BLNK (Phospho-Tyr84) antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | WB: 1:500 - 1:3000. IHC: 1:50 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
