Application
Western Blot analysis (reducing conditions) of whole cell lysate of Sp2/0 mouse myeloma cell line using polyclonal anti-mouse SLP65 / BLNK antibody .| Product Name | BLNK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BLNKBLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck. |
| Immunogen | A fusion protein representing amino acids 171-356 of human BLNK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at an assay dependent dilution. Predicted molecular weight: 54.7 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 604515, MGC111051, SLP65, Ly57, 29760, SLP-65, BASH, BLNK-s, BLNKs, BLNK, Q8WV28, SLP 65, BLNK s |
|---|---|
| Target | BLNK |
Application
Western Blot analysis (reducing conditions) of whole cell lysate of Sp2/0 mouse myeloma cell line using polyclonal anti-mouse SLP65 / BLNK antibody .| Application Notes | WB: Use at an assay dependent dilution. Predicted molecular weight: 54.7 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
