Application
Immunofluorescent staining of Bmf in human kidney tissue with Bmf antibody at 10μg/ml.Application
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with Anti-Bmf (Cat. No. ) at 2 mg /mL.| Product Name | BMF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BmfApoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor) (1). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program (1,2). Bmf is constitutively expressed in many tissues (1,2). |
| Immunogen | Synthetic peptide: ALNGEENRNGAGPR, corresponding to amino acids 171-184 of Human Bmf. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| This antibody can be used for detection of Bmf by Western blot at 2 µg/ml. HepG2 or 293 cell lysate can be used as a positive control and a band at approximately 25 kDa can be detected. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 90427, FLJ00065, 606266, Q96LC9, BMF |
|---|---|
| Target | BMF |
Application
Immunofluorescent staining of Bmf in human kidney tissue with Bmf antibody at 10μg/ml.
Application
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with Anti-Bmf (Cat. No. ) at 2 mg /mL.| Application Notes | This antibody can be used for detection of Bmf by Western blot at 2 µg/ml. HepG2 or 293 cell lysate can be used as a positive control and a band at approximately 25 kDa can be detected. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide |
